Trans-ABySS 2.0.1

Basic information

Installation

  1. Before installing Trans-ABySS install ABySS and BLAT. Then run:

    $ module load python/3.6.5_miniconda-4.5.1
    
    # Instead of "biology" any name can be used.
    $ conda create -n biology python=3.7
    $ conda activate biology
    $ pip install python-igraph
    

    Test:

    $ module load blat/36_intel-19.0.4
    $ module load abyss/2.2.3_gcc-5.4.0
    $ git clone https://github.com/bcgsc/transabyss.git
    $ cd transabyss
    $ bash sample_dataset/assemble.sh
    $ rm -rf sample_dataset/merged.fa  sample_dataset/test.k25  sample_dataset/test.k32
    

    Install the scripts. From the Trans-ABySS’ directory:

    $ sudo mkdir -p /share/apps/transabyss/2.0.1/miniconda-4.5.1
    $ sudo cp -r * /share/apps/transabyss/2.0.1/miniconda-4.5.1/*
    
  2. Create and place the needed module file. Create a file with the following content:

    #%Module1.0####################################################################
    ##
    ## module load transabyss/2018
    ##
    ## /share/apps/modules/transabyss/2018
    ## Written by Hamilton Tobon Mosquera.
    ##
    
    proc ModulesHelp {} {
             global version modroot
             puts stderr "Sets the environment for using Trans-AByss 2018 update \
             \nin the shared directory \
             \n/share/apps/transabyss/2.0.1/miniconda-4.5.1"
    }
    
    module-whatis "(Name________) transabyss"
    module-whatis "(Version_____) 2.0.1 Miniconda 4.5.1"
    module-whatis "(System______) x86_64-redhat-linux"
    module-whatis "(Libraries___) "
    
    # for Tcl script use only
    set         topdir        /share/apps/transabyss/2.0.1/miniconda-4.5.1
    set         version       2.0.1
    set         sys           x86_64-redhat-linux
    
    conflict transabyss
    module load blat/36_intel-19.0.4
    module load abyss/2.2.3_gcc-5.4.0
    
    prepend-path   PATH            $topdir
    prepend-path   PATH            $topdir/bin
    

    Create the needed folder and place it:

    $ sudo mkdir /share/apps/modules/transabyss
    $ sudo mv 2.0.1_miniconda-4.5.1 /share/apps/modules/transabyss/
    

Usage

Always activate the conda environment where python-igraph was installed, in this case:

conda activate biology

Authors